研究领域

通过蛋白质工程的方法研究泛素降解途径

泛素途径中的药物靶点发现和药物分子高通量筛选

泛素在癌症或疾病的发病过程中的调控机理

泛素相似蛋白Nedd8和SUMO在细胞中的调控机理

论文

1. Phage selection assisted by Sfp phosphopantetheinyl transferase catalyzed site-specific protein labeling.Bo Zhao, Keya Zhang, Karan Bhuripanyo, Han Zhou, Yiyang Wang, Mengnan Zhang, Jun Yin.Methods in Molecular Biology.

2. SUMO-mimicking peptides inhibiting protein SUMOylation.Bo Zhao, Eric Villhauer, Karan Bhuripanyo, Hiroaki Kiyokawa, Hermann Schindelin, Jun Yin.Chembiochem.

3. Profiling the Cross Reactivity of Ubiquitin with the Nedd8 Activating Enzyme by Phage Display.Bo Zhao, Keya Zhang, Karan Bhuripanyo, ChanHee Choi, Eric Villhauer, Heng Li, Ning Zheng, Hiroaki Kiyokawa, Hermann Schindelin, Jun Yin.PLoS One.

4. Phage Display to Identify Nedd8-Mimicking Peptides as Inhibitors of the Nedd8 Transfer Cascade.Bo Zhao, Keya Zhang, Eric Villhauer, Karan Bhuripanyo, Hiroaki Kiyokawa, Hermann Schindelin, Jun Yin.Chembiochem.

5. Engineering new Protein-Protein Interactions on the β-Propeller Fold by Yeast Cell Surface Display.Keya Zhang, Heng Li, Karan Bhuripanyo, Bo Zhao, Tiffany Chen, Ning Zheng, Jun Yin.Chembiochem.

6. Engineering the substrate specificity of the DhbE adenylation domain by yeast cell surface display.Keya Zhang, Kathryn Nelson, Karan Bhuripanyo, Kimberly Grimes, Bo Zhao, Courtney Aldrich, Jun Yin.Chemistry & Biology.

7. Orthogonal Ubiquitin Transfer through Engineered E1-E2 Cascades for Protein Ubiquitination.Bo Zhao, Karan Bhuripanyo, Keya Zhang, Hiroaki Kiyokawa, Hermann Schindelin, Jun Yin.Chemistry & Biology.

8. Inhibiting the Protein Ubiquitination Cascade by Ubiquitin-Mimicking Short Peptides.Bo Zhao, Chan Hee J. Choi, Karan Bhuripanyo, Eric B. Villhauer, Keya Zhang, Hermann Schindelin, Jun Yin.Organic Letters.

9. Specificity of the E1-E2-E3 Enzymatic Cascade for Ubiquitin C-terminal Sequences Identified by Phage Display.Bo Zhao, Karan Bhuripanyo, Jeffrey Schneider, Keya Zhang, Hermann Schindelin, David Boone, Jun Yin.Chemical Biology.